10mg Package
Recombinant Protein L

Formulation: Lyophilized Salt-free powder
Product#: L100L10
 
$159.00 USD each

Add to Cart

 

30mg Package
Recombinant Protein L

Formulation: Lyophilized Salt-free powder
Product#: L100L30
 
$319.00 USD each

Add to Cart

 

Bulk Size
Recombinant Protein L

Formulation: Lyophilized Salt-free powder
multi-gram lots available
Available upon request

Bulk Quote

MPT Biotech
 
Frost & Sullivan 2012 Best Practices Award

Protein L

Recombinant Protein L, E. coli-expressed

Protein L is an immunoglobulin-binding protein originally derived from the surface of bacterium Peptostreptococcus magnus. Protein L binds to Ig through L chain interaction from which the name was derived. The native Protein L consists of 719 amino acid residues with the molecular weight estimated as 95 kDa by SDS-PAGE analysis. Protein L does not contain any inter chain disulfide loops, nor does it consist of disulfide-linked subunits. This version of Protein L is now produced recombinantly in our unique MPTxpress liquids-free, fully single-use E.coli manunfacturing platform.

Protein L is available in bulk, including for 3rd party manufacture of resins. Please contact us for further information.

Protein Description

Recombinant Protein L is expressed by MicroProtein Technologies in E.coli using its MPTxpress upstream platform with innovative purification methods. MicroProtein's Recombinant Protein L is a non-glycosylated polypeptide having a molecular mass of 41 kDa and comprised of 5 IgG-binding regions of Protein L (B1-B5). The cell wall-binding region, cell membrane-binding region and albumin-binding regions have been eliminated from this version of recombinant Protein L to ensure maximum specific IgG binding. Protein L has the unique ability to bind through kappa light chain interaction without interfering with an antibody antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes compared to Proteins A, G, etc. Protein L will bind to all classes of Ig (IgA, IgD, IgE. IgG and IgM). Protein L will also bind single chain variable fragment (scFV), Fab, and Dab fragments. Protein L binds kappa 1, 3, 4 in general, making it optimal for purification of many newer engineering antibodies.

Recombinant Protein L is valuable as the basis for preparing various kinds of affinity media or probes for detection or purification of human and mouse antibodies in immunoassays and antibody purification protocols.

See the Recombinant Protein L Data Sheet.

Source

Escherichia coli cultured using the MPTxpress platform. (Apparent MW by SDS-PAGE = 41,000 Da)

Store

Store at 4°C if the entire vial will be used within 5 weeks. Store, frozen at -20°C for longer periods of time. After reconstitution in distilled water or saline, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

Purity and Formulation

≥95% by SDS-Page and HPLC analyses. Lyophilized with no additives

Usage

MicroProtein's material is furnished for laboratory or bioprocessing purposes, including manufacture of Protein L resins. The product may not be used for therapeutic or diagnostic purposes for humans or animals.

Purchase

10mg Package
Recombinant Protein :

Formulation: Lyophilized Salt-free powder
Product#: L100L10
 
$159.00 USD each

Add to Cart

 

30mg Package
Recombinant Protein L

Formulation: Lyophilized Salt-free powder
Product#: L100L30
 
$319.00 USD each

Add to Cart

 

Bulk Size
Recombinant Protein L

Formulation: Salt-free powder
grams and larger lots available.
Prices upon request

Bulk Quote