After years of proprietary research and development, MicroProtein Technologies Inc. has developed a robust solid media-based E. coli manufacturing platform, MPTxpress, which far surpasses conventional fermentation as a reliable and inexpensive way to produce recombinant proteins.

MPTxpress has advantages including higher yields with incredible simplicity and related cost savings, including no need for bioreactors, mixers, etc.; is fully singleuse; minimizes up-stream and down-stream processing; and eliminates the need for expensive fixed stainless steel equipment. E. coli is cultured on top of a membrane laid on top of 12-inch square agar plates, with harvesting involving scraping concentrated protein paste off the membrane. Each plate produces >1 gram of protein after incubation overnight at room temperature in a standard lab incubator. MPTxpress is scalable to commercial manufacture, e.g., ≥1 kg/day – simply use more trays (mini bioreactors in parallel)

Read more about our novel upstream and fermentation process in our feature in BioProcess International here and in Genetic Engineering Biotechnology News here.