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913-851-0877 info@mptbiotechs.com
913-851-0877 info@mptbiotechs.com
The gene we selected to use for the production of Protein G originated from Streptococcus Strain G148, and was cloned and expressed in our proprietary strain of Escherichia coli. The regions of the gene corresponding to the albumin binding domains, as well as the cell wall and cell membrane binding domains, have been genetically deleted, however the Fc binding domain is still present. Our recombinant Protein G contains 196 amino acid residues and has a predicted molecular mass of approximately 22.5 kDa, but migrates with an apparent molecular mass of about 30 kDa in SDS-PAGE.
Animal component free (ACF) and Animal origin free (AOF)
G5: 2ug of recombinant Protein G resolved by SDS-PAGE reducing condition, showing a single band of 22.5 kDa.
Recombinant Streptococcus Protein G is produced in E. coli and is a non-glycosylated polypeptide chain containing 196 amino acids. Streptococcus Protein G is proprietarily produced in E. coli and purified via chromatographic techniques. The product obtained is consistent in quality and yield because our proprietary bacterial platform is engineered to be deficient of major proteolytic activities. Protein G binds through a non-immune mechanism specifically to the Fc region of IgG and to all the human subclasses. It also has a strong affinity to monoclonal mouse IgG1 and rat IgG.
MicroProtein Technologies Inc Protein G is furnished for laboratory or bioprocessing purposes. The product may not be used as a drug for humans or animals.
Lyophilized without a carrier.
≥95% by SDS PAGE and HPLC analysis.
Escherichia coli. cultured using MPTxpress platform
Store at 4°C if entire vial will be used within 5 weeks. Store, frozen at -20°C for longer periods of time. After reconstitution in distilled water or saline, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Formulation: Lyophilized Salt-free powder
Product#: G100L20
Formulation: Lyophilized Salt-free powder
Product#: G100L50
Formulation: Salt-free powder
grams and larger lots available.
Escherichia coli
Store at 4°C if entire vial will be used within 5 weeks. Store, frozen at -20°C for longer periods of time. After reconstitution in distilled water or saline, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
≥95% by SDS-Page and HPLC analyses. Lyophilized with no additive.
MicroProtein’s material is furnished for laboratory or bioprocessing purposes. The product may not be used as a drug for humans or animals.
Formulation: Lyophilized Salt-free powder
Product#: G100L20
Formulation: Lyophilized Salt-free powder
Product#: G100L50
Formulation: Salt-free powder
grams and larger lots available.
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