Fibroblast Growth Factor-Basic 146 (rhFGFb-146) is a member of the FGF family of 23 related proteins known to be involved in angiogenesis, wound healing, embryonic development and differentiation, and neuronal function and neural degeneration.  As a family, they bind to heparin and signal through four receptor tyrosine kinases called FGFR1, 2, 3, and 4.  FGFb stimulates the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal and endodermal origin.

When added to cell culture media, FGFb is capable of maintaining human stem cells long term in feeder and feeder-free conditions.  As such, stem cells will remain in an undifferentiated state in the expansion and maintenance phase until later when differentiation to various types of cells is triggered by adding various combinations of growth factors. 

MicroProtein Technologies (MPT) Fibroblast Growth Factor-Basic (FGFb) is produced using MPT’s novel and innovative E. coli based solid media platform, MPTxpress.  The final product is a Pro143-Ser288, non-glycosylated protein containing 146 amino acids with an approxiamte molecular weight of 16.5kDa.

This product is Animal component free (ACF) and Animal origin free (AOF)

Activity

• MicroProtein Technologies’ rhFGFb-146 activity on NIH 3T3 cell growth
  
• Cell proliferation figures 48hours and 72hours incubation respectively

Endotoxin Level

<0.1 EU per µg of protein using LAL analysis.

Formulation

Lyophilized in 20mM Tris-HCL and 1M NaCl.

Purity

97% by SDS PAGE and HPLC analysis.

Amino Acid Sequence

PALPEDGGSG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH IKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTD ECFFFERLES NNYNTYRSRK YTSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS

Source

Expressed in Escherichia coli. using MPTxpress platform.

Reconstitution

at 100-250µg/mL in sterile PBS.

Storage

Store at -20°C to -70°C as supplied, protected from light and moisture; Shelf life: 1 year from the date of receipt.
After reconstitution, the product may be stored for a maximum of two weeks at 2-8°C or may be stored in aliquots at -20°C for a maximum of 6 months.