Recombinant Protein L

Recombinant Protein L is expressed by MicroProtein Technologies in E.coli using its MPTxpress upstream platform with innovative purification methods. MicroProtein’s Recombinant Protein L is a non-glycosylated polypeptide having a molecular mass of 41 kDa and comprised of 5 IgG-binding regions of Protein L (B1-B5). The cell wall-binding region, cell membrane-binding region and albumin-binding regions have been eliminated from this version of recombinant Protein L to ensure maximum specific IgG binding. Protein L has the unique ability to bind through kappa light chain interaction without interfering with an antibody antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes compared to Proteins A, G, etc. Protein L will bind to all classes of Ig (IgA, IgD, IgE. IgG and IgM). Protein L will also bind single chain variable fragment (scFV), Fab, and Dab fragments. Protein L binds kappa 1, 3, 4 in general, making it optimal for purification of many newer engineering antibodies.

Recombinant Protein L is valuable as the basis for preparing various kinds of affinity media or probes for detection or purification of human and mouse antibodies in immunoassays and antibody purification protocols.

See the Recombinant Protein L Data Sheet.

Applications

MicroProtein’s material is furnished for laboratory or bioprocessing purposes, including manufacture of Protein L resins. The product may not be used for therapeutic or diagnostic purposes for humans or animals.

Source

Escherichia coli cultured using the MPTxpress platform. 

Purity

≥97% by SDS-Page and HPLC analysis.

Formulation

Lyophilized with no additives, salt free.

Storage

Store at 4°C if the entire vial will be used within 5 weeks. Store, frozen at -20°C for longer periods of time. After reconstitution in distilled water or saline, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.